87312_Ramos-24-h-stim(Cy5)-+-Ramos-24-h-control(Cy3)-+-Stnd(Cy2)

2D DIGE image overlays enable easy visual comparison between two protein samples labeled with Cy3 and Cy5 and presented in green and red colors. The more mature B cell type is presented in red color. Triple image overlays enable comparison between the two samples in relative to internal standard sample (Stnd) labeled with Cy2 and presented in blue color.

Explanation for colors in double image overlays:
Yellow: Proteins shared between the two samples.
Green and red: Unique proteins in the sample. In Ramos samples (Gels 5-7) red indicates up-regulation and green down-regulation in response to anti-IgM stimulation.

Triple image overlays:
White: Proteins shared between the three samples (no difference in protein abundance).
Yellow: Proteins shared between the two samples. Protein abundance is high in the two samples in relative to Stnd.
Blue: Protein abundance is negligible in relative to Stnd.
Green and red: Unique proteins in the sample. Protein abundance is high in relative to Stnd and to the other sample.
Turquoise and purple: Protein abundance is higher/lower in relative to the other sample.